Specimen Collection Checklist

A helpful guide on how to improve your specimen collection workflow.

This checklist helps healthcare organizations evaluate whether their collection practices consistently protect specimen integrity, or if hidden gaps could lead to diagnostic errors, redraws, and delays.

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Purpose of This Checklist

This checklist provides a structured framework for assessing the safety, consistency, and compliance of specimen collection workflows. It helps teams identify vulnerabilities in processes, workflow, and documentation before they result in patient harm or operational disruption.

 

How it Guides Hospital Teams:

  • Evaluate labeling and identification practices to eliminate misidentification risk
  • Identify workflow gaps that may contribute to contamination, timing errors, or specimen rejection
  • Strengthen documentation and communication to improve regulatory readiness

 

Intended Audience and Usage

This checklist is designed for laboratory leaders, nursing leadership, phlebotomy supervisors, quality teams, and compliance professionals responsible for specimen integrity. It can be used to conduct internal gap assessments, prepare for accreditation surveys, or support quality improvement initiatives. For best results, it should be completed collaboratively across laboratory and clinical departments to ensure a comprehensive review of current practices.

Expand the Checklists Below:

Patient Safety and Satisfaction

Patient and Specimen Identification

  • Two patient identifiers are verified verbally and/or against the wristband prior to collection to ensure accuracy.
  • Patient wristband barcode scanning is used as an enhanced safety feature.
  • Specimens are labeled in the presence of the patient.
  • The specimen is matched to the order before leaving the bedside.
  • Specimen label barcode scanning is used during collection for specimen verification.
  • A protocol exists for handling identifier mismatches (patient and specimen).
  • Misidentification events are reported promptly and reviewed for process improvements.

Timeliness of Specimen Collection 

  • STAT collections are communicated to caregiver in real time to ensure prompt collection.
  • STAT collections can be easily identified by the caregiver to ensure prompt collection.
  • Timed draws can be easily identified by the caregiver to ensure collections are performed at the ordered time.
  • Priority lab tests are easily identified by the caregiver to enable the caregiver to integrate these into their workflow appropriately.

Special Collection Alerts

  • Information indicating the correct order of draw is readily available during collection.
  • Specimen collection instructions are provided at the time of collection (i.e., if the specimen needs to be placed on ice).
  • Patient status requirements are viewable at the time of collection (i.e., Fasting).

Repeat and Unnecessary Venipunctures

  • Current and future orders are easily viewable during the collection process for the caregiver to consider all collections that can be completed simultaneously.
  • Past and future specimen collection orders are easily viewed during the collection process for caregivers to consider if there is an opportunity for an “add-on” lab.
  • A defined process exists for managing add-on orders.

Necessary Label Information

  • Two patient identifiers (e.g., full name and DOB or MRN).
  • Order test and order number.
  • Barcode of order number.
  • Container type.
  • Collection date and time.
  • Caregiver identification.
  • Stat and Timed specimen label identifier.

Mislabeling

  • Specimen labels are printed at the time and location of collection to ensure accuracy.
  • Wrong patient alerts are provided during patient verification to eliminate incorrect collections.
  • Wrong specimen alerts are provided during specimen verification, to eliminate specimen labeling errors.
  • Tubes are never pre-labeled prior to collection to avoid mislabeling errors.
  • Unreadable or damaged labels are rejected and replaced appropriately to avoid mislabeling errors and/or incorrect collection documentation.
  • A defined process exists for managing relabeling errors or mislabeled specimens.
The Collection Workflow Process

Workflow Efficiency

  • Caregiver can easily identify which containers will be needed for collection.
  • STAT collections are communicated to caregiver in real time to ensure prompt collection.
  • Collection list can be individualized to meet the needs of the caregiver.
  • Past and future specimen collection orders are easily viewed during the collection process.
  • Specimen collection instructions are provided at the time of collection to stay top of mind for caregivers.
  • Electronic method of communication with other team members regarding collections is readily available (i.e., unable to collect).
  • Method of documentation of collection activities (not collected reasons, collection comments) is readily available during a collection to ensure accurate reporting and follow up.
Necessary Documentation and Reporting

Documentation Considerations

  • Date and time of collection are documented accurately and legibly at the time of collection.
  • Caregiver identification is documented accurately and legibly at the time of collection.
  • Collection site is documented when required.
  • Unsuccessful attempts, patient refusals and other reasons specimens are not collected are documented.
  • Other collection events are documented as required (i.e., extra tubes collected).

Reporting Considerations

  • Full specimen traceability is possible (collection → labeling → user → timestamp) to ensure patient safety through caregiver accountability.
  • Adherence to expected processes can be monitored, while incorrect or incomplete processes are noted for workflow improvements.
  • Not collected reasons can be monitored.
  • Use of demographic labels instead of specimen specific labels can be monitored.
  • Extra tube collections can be monitored.
  • Patient and specimen identification errors can be monitored.
  • Patient and specimen verification method can be monitored.
  • Specimen turnaround times/productivity can be monitored.

For more information about specimen collection, and to speak with an expert about reviewing your collection process today:

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